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dc.creatorFIGUEROA ESPI, V.-
dc.creatorALVAREZ PANEQUE, A.-
dc.creatorTORRENS, M.-
dc.creatorOTERO GONZALEZ, A.J.-
dc.creatorREGUERA, E.-
dc.date2012-03-23T00:24:01Z-
dc.date2012-03-23T00:24:01Z-
dc.date2011-08-11-
dc.date.accessioned2013-01-16T09:52:41Z-
dc.date.available2013-01-16T09:52:41Z-
dc.date.issued2013-01-16-
dc.identifierColloids and Surfaces A: Physicochem. Eng. Aspects 387 (2011) 118– 124-
dc.identifierhttp://hdl.handle.net/123456789/284-
dc.identifier.urihttp://www.repositoriodigital.ipn.mx/handle/123456789/10779-
dc.descriptionIn this study the potential applications of manganese ferrite (MnFe2O4) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe2O4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation. © 2011 Elsevier B.V. All rights reserved.-
dc.languageen_US-
dc.publisherColloids and Surfaces A: Physicochemical and Engineering Aspects-
dc.subjectNanoparticles bioconjugate-
dc.subjectNanoparticles immunoassay-
dc.subjectSticholysin-
dc.subjectProtein separation-
dc.titleConjugation of manganese ferrite nanoparticles to an anti Sticholysin monoclonal antibody and conjugate applications-
dc.typeArticle-
Aparece en las colecciones: Doctorado

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