Diagnóstico de Mycoplasma hominis y Ureaplasma urealyticum en neonatos de unidad de cuidados intensivos del INPer IER, mediante una prueba de detección rápida en secreción bronquial, comparada con medios de cultivo y reacción en cadena de polimerasa

Abstract

Ureaplasma urealyticum and Mycoplasma hominis its microorganisms implied in neonatal and maternal diseases. These agents have been in different newborn organs. Both are important cause of pneumonia in the newborn ones exposed to factors of risk in units of neonatal intensive cares. These bacteria in laboratory frequently require means of culture supplemented to obtain the growth, which has made difficult the diagnostic confirmation. The culture of bronchial secretions in solid media and liquids has been a valuable tool of diagnosis. Nevertheless, the time in which grants results is prolonged, which is translated in the delayed beginning of antimicrobial handling and increase in the morbimortalidad. At the moment alternative methods of diagnosis of these bacteria based on the microbial metabolism exist, inmunoenzimáticos tests of molecular Biology, tests and serologic tests. This study took place in the National Institute of Perinatology, in the department of Infectology and Perinatal Immunology, was used a test of fast detection (Mycofast Evolution 3), as alternative diagnostic to detect Mycoplasma hominis and Ureaplasma urealyticum in samples of aspired bronchial of newborn intubated of UCIN, which were inoculated with stocks of reference of both bacteria in order to value if this test is able to detect both bronchial secretion microorganisms, since there are no antecedents in Literature, of which has been used in this type of secretion. The samples also inoculated were put under studies of Biology molecular (PCR) and seeded in traditional means of culture (urea broth or broth arginine) to detect agreement of obtained positive and negative results with the fast test. This study had as an aim to compare the results obtained with the gold standard. It constitutes a study of diagnostic test, in which inoculated samples of aspired bronchial were analyzed with reference stocks, by 3 different methods diagnoses: Mycofast evolution 3, chain reaction of polymerase and means of culture. Sensitivity, specificity, positive predictive value, negative predictive value and agreement between the 3 methods were determined.