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Título : | Nucleolin interacts with the Feline calicivirus 3’ untranslate region and the protease-polymerase NS6 and NS7 proteins, playing a role in virus replication |
Autor : | Gutiérrez-Escolano, Ana Lorena Cancio-Lonches, Clotilde Yocupicio-Monroy, Martha Sandoval-Jaime, Carlos Galván-Mendoza, Iván Ureña, Luis Vashist, Surender Goodfellow, Ian Salas-Benito, Juan Santiago Gutiérrez-Escolano, Ana Lorena |
Palabras clave : | Calicivirus Nucleolina Replicación Transporte nuclear Proteasa-polimerasa |
Fecha de publicación : | ago-2011 |
Editorial : | Journal of Virology |
Citación : | Cancio-Lonches C, Yocupicio-Monroy M, Sandoval-Jaime C, Galván-Mendoza I, Ureña L, Vashist S, Goodfellow I, Salas-Benito J, Gutiérrez-Escolano AL. Nucleolin interacts with the Feline calicivirus 3’ untranslate region and the protease-polymerase NS6 and NS7 proteins, playing a role in virus replication. J Virol. 2011. 85(16): 8056-8068. |
Resumen : | Cellular proteins play many important roles during the life cycle of all viruses. Specifically, host cell nucleic acid-binding proteins interact with viral components of positive-stranded RNA viruses and regulate viral translation, as well as RNA replication. Here, we report that nucleolin, a ubiquitous multifunctional nucleolar shuttling phosphoprotein, interacts with the Norwalk virus and feline calicivirus (FCV) genomic 3 untranslated regions (UTRs). Nucleolin can also form a complex in vitro with recombinant Norwalk virus NS6 and -7 (NS6/7) and can be copurified with the analogous protein from feline calicivirus (p76 or NS6/7) from infected feline kidney cells. Nucleolin RNA levels or protein were not modified during FCV infection; however, as a consequence of the infection, nucleolin was seen to relocalize from the nucleoli to the nucleoplasm, as well as to the perinuclear area where it colocalizes with the feline calicivirus NS6/7 protein. In addition, antibodies to nucleolin were able to precipitate viral RNA from feline calicivirus-infected cells, indicating a direct or indirect association of nucleolin with the viral RNA during virus replication. Small interfering RNA (siRNA)-mediated knockdown of nucleolin resulted in a reduction of the cytopathic effect and virus yield in CrFK cells. Taken together, these results demonstrate that nucleolin is a nucleolar component that interacts with viral RNA and NS6/7 and is required for feline calicivirus replication |
Descripción : | Artículo científico |
URI : | http://www.repositoriodigital.ipn.mx/handle/123456789/7311 |
ISSN : | 0022-538X |
Aparece en las colecciones: | Artículos |
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