Efecto de prebióticos y microorganismos con potencial probiótico en el crecimiento y supervivencia de la tilapia Oreochomis niloticus, cultivada en condiciones experimentales

Abstract

The effect of inulin, fulvic acid, lactic acid bacteria (LAB) and yeasts on growth and survival of tilapia Oreochromis niloticus was determined. LAB and yeasts were isolated from the intestine of tilapia. We used inulin from tequila agave and fulvic acid from Fertichem. Characterization was performed (Gram stain, morphology, cell arrangement, hemolytic activity, antagonism, growth, hydrophobicity, extracellular enzyme activity, counting colony forming units) to select those microorganisms with probiotic potential. Three bioassays were performed in triplicate in plastic tanks with 800 L of filtered fresh water and 12 organisms per tank of about 1 g. The aeration was constant. Bioassay 1: I) Control (commercial feed), II) 5 g of inulin/kg feed; III) 5 g of fulvic acid/kg feed; IV) 2.5 g of inulin + 2.5 g fulvic acid/kg feed. Bioassay 2: I) Control (commercial feed); II) BAL (5 x 104 CFU/g feed) + 2.5 g inulin + 2.5 g fulvic acid/kg feed; III) BAL (2.5 x 105 CFU/g feed) + 2.5 g inulin + 2.5 g fulvic acid/kg feed; IV) BAL (5 x 105 CFU/g feed) + 2.5 g inulin + 2.5 g fulvic acid/kg feed. Bioassay 3: I) Control (commercial feed); II) Yeast (5 x 104 CFU g feed) + 2.5 g inulin + 2.5 g fulvic acid/kg feed; III) Yeast (2.5 x 105 CFU/g feed) + 2.5 g inulin + 2.5 g fulvic acid/kg feed; IV) Yeast (5 x 105 CFU/g feed) + 2.5 g inulin + 2.5 g fulvic acid/kg feed. Survival was 100% in the 3 bioassays. A significantly higher growth, as compared with control group, was observed in the bioassay 2 when tilapias were fed with BAL (2.5 x 105 CFU/g feed) and prebiotics (2.5 g fulvic acid + 2.5 g inulin).