DIFERENCIAS DE LAS FRECUENCIAS DE LOS POLIMORFISMOS DEL CYP3A4*1B Y CYP3A5*3,*6 Y *7 ENTRE AMERINDIOS HUICHOLES, CORAS Y TARAHUMARAS

Abstract

Have been reported many genetic polymorphisms that affect the functionality of the CYP450 enzymes, which are a major cause of adverse effects or therapeutic failure by drugs.CYP3A4 and CYP3A5 are the most important isoforms in drug metabolism and more abundant in the liver and intestine. Between polymorphisms that severely reduced the synthesis of these enzymes and affect the hydroxylation of different drugs are CYP3A4* 1B y CYP3A5* 3, * 6 and * 7. These variants have been studied in different ethnic groups, but few studies in the indigenous population. The objective of this study was to identify differences in the frequencies of polymorphisms between Amerindians Huichol, Cora and Tarahumara. We studied 50 individuals from each group. The determination of the polymorphisms was performed by PCR with Taqman probes. The frequency of CYP3A4* 1B was 0.01 in Huichol and Cora, while in Tarahumara was 0.04. For CYP3A5* 3 frequencies were 0.06, 0.14 and 0.32 in Huichol, Cora and Tarahumara, respectively. CYP3A5* 6 was identified only Cora and Tarahumara with a frequency of 0.01 and CYP3A5* 7 allele was not found in any group. The differences in allele frequencies were not statistically significant. The frequencies of the CYP3A4 * 1B are lower than those observed in Tepehuanos of Durango (0.08) and other indigenous groups in southern Mexico, and equally low for the allele CYP3A5 * 3 in comparison with Caucasians (0.85), Hispanics (0.76 ) And Asians (0.71).