ASOCIACIÓN DE LOS POLIMORFISMOS DE INTERLEUCINA-1 BETA (+3953) Y FACTOR DE NECROSIS TUMORAL ALFA (-308), EN PACIENTES CON RUPTURA PREMATURA DE MEMBRANAS AMNIÓTICAS

Abstract

Preterm delivery, occurs approximately in 6 to 8% of women before 37 weeks gestation, it is a direct antecedent of 20 to 50% of all preterm births. Particulary, some cytokines, as IL-1 ß and TNF -308 have been implicated in the induction of preterm births. Therefore determine if polymorphisms are involved in PPROM and wich of them. Identify a polymorphism could be use as indicator to identify women who need an early and specific management, thus helping to reduce perinatal mortality. OBJECTIVE Determining association frequency between polymorphisms A2 allele of IL-1ß +3953 and TNF -308, and pregnancies with preterm RPM. DESIGN AND METHODOLOGY This case control study was conducted in the Department of Infectious Diseases and Immunology, and the Toco-surgical unit of National Institute of Perinatology. The population study consisted in patients of pregestational risk clinic, hospitalization unit and outpatient area. Identification of allelic polymorphism IL-1ß and TNF a was carried out using the PCR technique. RESULTS The total frequency of the polymorphic A2 allele of IL-1ß +3953 was lower in women with RPM (0.44 vs. 0.71), while the A1 allele was more frequent in the group with RPM (0.55 vs. 0.29). Significant difference between groups (p <0.05) was observed. On the other hand the frequency of the A1 allele of -308 TNF a was lower in women with and without RPM, while the A2 allele frequency was high in both groups (0.93 and 0.90), yet there weren’t significant differences between groups p> 0.05. CONCLUSIONS Carriage of A1 allele of IL-1ß +3953 is a risk factor for develop neonatal sepsis and PPROM, while carrying A2 allele of IL-1ß +3953 has protective effect for against develop sepsis or PPROM. There was no difference between being carriers of the polymorphism of TNF a -308 allele A2 and the PPROM.