COMPARACIÓN DE DOS MÉTODOS PARA OBTENER ADN TOTAL DE Phaseolus vulgaris PARA ANÁLISIS DE ISTR

Abstract

Two CTAB methods to isolate DNA from foliar tissues of bean Pinto Saltillo were modified and compared. Differences in the tissue grinding, the isolation buffers composition, and in the number of wash steps of genetic material resulted in significant differences in the yield and purity of DNA isolated. From the DNA obtained by the method, which allowed obtaining the highest amount (3996.249 ng/µL) and purity (A260/A280 = 1.68) of DNA, ISTR sequences were amplified by PCR.