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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Escobedo Bonilla, César M. | - |
dc.contributor.author | Esparza Leal, Héctor M. | - |
dc.contributor.author | Casillas Hernández, Ramón | - |
dc.contributor.author | Álvarez Ruíz, Píndaro | - |
dc.contributor.author | Portillo Clark, Guillermo | - |
dc.contributor.author | Valerio García, Roberto C. | - |
dc.contributor.author | Hernández López, Jorge | - |
dc.contributor.author | Méndez Lozano, Jesús | - |
dc.contributor.author | Vibanco Pérez, Norberto | - |
dc.contributor.author | Magallón Barajas, Francisco J. | - |
dc.date.accessioned | 2012-09-28T20:28:32Z | - |
dc.date.available | 2012-09-28T20:28:32Z | - |
dc.date.issued | 2009-07-01 | - |
dc.identifier.uri | http://www.repositoriodigital.ipn.mx/handle/123456789/7101 | - |
dc.description.abstract | White spot syndrome virus (WSSV) may spread through water to neighbor ponds or farms. Routine water exchange and wastewater released during white spot disease (WSD)-emergency harvests may preserve WSSV in shrimp farming areas. To test this hypothesis, on-site experiments were performed in a WSSV-affected farm in Guasave, Sinaloa, Mexico. Plankton and shrimp hemolymph were collected from 12 ponds during a WSD outbreak. PCR analyses showed that 72% of the hemolymph pools (26 out of 36) were WSSV-positive. In contrast, only 14% (4 of 28) plankton samples (filtered through 10 and 0.45 µm) from three ponds (2, 7 and 10) were WSSV-positive. Plankton from pond 9 was WSSV-negative, but 14 days later, shrimp began to die. At this point, a differential filtration experiment was performed in pond 9. WSSV-positive samples were only found in three fractions [particulate fraction (PF) 1 µm and liquid fractions (LF) < 100 and < 40 µm]. Both LFs and PFs were used for insitu infectivity assays by water-borne routes in WSSV-negative whiteleg shrimp Penaeus (Litopenaeus) vannamei. Some shrimp exposed to different PFs and LFs (100 µm to > 0.65 µm) became WSSV-positive. Results indicate that water fractions between 100 and 0.65 µm induced WSSV infection to shrimp. Results showed that pond water and/or particulate fractions are vehicles for WSSV dispersion via virus suspended in water, attached to microalgae, or carried by zooplankton. | es |
dc.language.iso | en | es |
dc.publisher | http://www.sciencedirect.com | es |
dc.subject | Infectivity assay | es |
dc.subject | Pond water | es |
dc.subject | Differential filtration | es |
dc.subject | Shrimp farm | es |
dc.subject | WSSV | es |
dc.subject | Penaeus (Litopenaeus) vannamei | es |
dc.title | Detection of white spot syndrome virus in filtered shrimp-farm water fractions and experimental evaluation of its infectivity in Penaeus (Litopenaeus) vannamei | es |
dc.type | Article | es |
dc.description.especialidad | Investigación | es |
dc.description.tipo | 7 pages | es |
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Fichero | Descripción | Tamaño | Formato | |
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Detection of white spot syndrome virus in filtered shrimp.pdf | 172.69 kB | Adobe PDF | Visualizar/Abrir |
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