Por favor, use este identificador para citar o enlazar este ítem:
http://repositoriodigital.ipn.mx/handle/123456789/7078
Título : | Double-stranded RNA against white spot syndrome virus (WSSV) vp28 or vp26 reduced susceptibility of Litopenaeus vannamei to WSSV, and survivors exhibited decreased susceptibility in subsequent re-infections |
Autor : | Escobedo Bonilla, César Marcial Mejía Ruíz, Claudio Humberto Vega Peña, Sergio Alvarez Ruiz, Píndaro |
Palabras clave : | dsRNA Inhibition of virus replication Extended susceptibility reduction Consecutive WSSV inoculations Litopenaeus vannamei |
Fecha de publicación : | may-2011 |
Editorial : | http://www.sciencedirect.com |
Resumen : | Abstract The antiviral effect of vp28 or vp26 double-stranded (ds) RNA upon single or consecutive white spot syndrome virus (WSSV) intramuscular challenges with a high infectious dose was evaluated. The vp28 dsRNA showed the highest protection both in single (LT50 = 145 h at 10 d and 98 h at 20 d post treatment [dpt]) or consecutive (LT50 = 765 h) WSSV challenges compared to vp26 dsRNA (LT50 = 126 h at 10 d and 57 h at 20 dpt vs. consecutive challenge LT50 = 751 h). Single WSSV challenges showed that animals treated with vp28 or vp26 dsRNA gradually lost the antiviral effect as virus challenge occurred at 10 dpt (cumulative mortality 63% vs. 80%, respectively) or 20 dpt (87% vs. 100%, respectively). In contrast, animals treated with vp28 or vp26 dsRNA and consecutively challenged with WSSV showed and extended lower susceptibility to WSSV. All dead animals were WSSV-positive by one-step PCR, whereas all surviving shrimp from single or continuous challenges were WSSV-negative as determined by reverse transcription (RT)-PCR. In conclusion, shrimp treated with a single administration of vp28 or vp26 dsRNA and consecutively challenged with WSSV showed a stronger and longer antiviral response than shrimp exposed once to WSSV at 10 or 20 dpt. |
URI : | http://www.repositoriodigital.ipn.mx/handle/123456789/7078 |
Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Double-stranded RNA against white spot.pdf | 80.31 kB | Adobe PDF | Visualizar/Abrir |
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.