Evaluación de la activación endotelial inducido por TiO2. Un modelo de co-cultivos múltiple

Abstract

Every time we breathe we introduce air and particulates that are suspend in it. Nowadays industries are using different materials with nanometer size. In Mexico the main nanomaterial used is TiO2. People exposed to TIO2 are principally workers with direct contact to mentioned nanomaterial. In this study, we evaluated the effects induced by NPs-TiO2 in cell communication (epithelial and endothelial). We developed an “in vitro” model which resembles the alveolar-capilar interface. We assessed cell adhesion, release of proinflamamatory factors such as cytokines, TNF-α, IL-1β, IL-6, chemokine IL-8, nitric oxide and the expression of adhesion molecules. We found that NPs-TiO2 (10μg/cm2 concentration) activated endothelial cells through epithelial cells, which induced a significant increase in the expression of adhesion molecules and adhesion of monocytes in endothelial cells. The production of nitric oxide and release of TNF-α increased significantly. These results comprobe that when TiO2 nanoparticles are present they cause the release and expression of proinflammatory elements and adhesion of monocytes to endothelial cells. The observed endothelial activation suggest that this procdess could open the way to lymphocytes but simultaneously to the TiO2 nanoparticles that can enter the bloodstream and travel to internal organs of people exposed to titanium nanomaterial causing serious health problems.