COMPARACIÓN DE LOS POLIMORFISMOS -5312 C/T EN EL ENHANCER CORIÓNICO Y -11003 G/A EN EL ENHANCER RENAL DEL GEN REN ENTRE MUJERES CON PREECLAMPSIA-ECLAMPSIA Y EMBARAZO NORMO-EVOLUTIVO

Abstract

AIM: To determine the association of the chorionic enhancer -5312 C/T and kidney enhancer -11003 G/A polymorphisms of the REN Gene with preeclampsia-eclampsia (PEE). METHODS AND MATERIALS. Case-control design. Cases group was integrated by 50 preeclampsia-eclampsia patients, and the control group by 48 normoevolutive pregnancy women. The disease was classified and diagnosed according to Sibai´s criteria. The groups were matched by weeks of gestation, age and number of deliveries. Patients with underlying diseases such as lupus, diabetes, kidney diseases, chronic hypertension and tobacco consumption were excluded. Clinical data from studied population were collected as well as polymorphism analysis. The chorionic enhancer polymorphism -5312 C/T were evaluated through automated sequencing while -11003 kidney enhancer was assessed by RFLP analysis.. RESULTS. No association was found between kidney enhancer -11003 G/A polymorphism of the REN Gene with PEE. It was not possible to detect the polymorphism -5312 C/T of the chorionic enhancer; however a new nucleotide variation G/A in position -5320 was found, 8 bp upstream the original polymorphic position. This new variation did not showed significant differences between groups, neither haplotypes analyzed. Nevertheless, a trend for GG genotype in kidney enhancer was noticed in severe preeclamptic patients without reach statistic differences. CONCLUSIONS. The studied polymorphisms on REN gene enhancers were not associated with preeclampsia. A new polymorphism G>A, in position -5320 was found in our population.The homozygous state GG in kidney enhancer -110003, was found more frequently in severe affected patients. This trend make necessary to increase the studied population