Correlación entre las características morfológicas y el inmunofenotipo de los linfomas B difusos de células grandes que diseminan a la médula ósea

Abstract

The B lymphoma diffuse large cell (DLBCL) are a heterogeneous group of malignant tumors composed of large cells with diffuse growth pattern. They are divided into subgroups by their morphology, immunophenotype and its origin. Spread to bone marrow is from 11 to 27% and can be concordant or discordant with respect to the size of the neoplastic cell and could determine differences in patient survival. Objective: To determine the proportion of infiltration consistent with respect to immunophenotype. Material and methods: Samples from patients with histopathological diagnosis of DLBCL, monoclonal antibodies: CD 20, CD 2, CD 5, BCL2, BCL6, CD10 and Mum-1, Eber in patients older of 50 years and bone marrow biopsies from 2002 to 2010. Results: 108 cases, 59 were men (54.6%), the mean age was 51.9 years . The frequency of location was: lymph node (60.1%), palatine tonsil (13.%), ocular adnexa (6.5%), gastrointestinal tract (3.7%) and other (16.7%). The morphological distribution was: centroblastic (80.5%), anaplastic (10.1%) and immunoblastic at (9.2%). Bone marrow infiltration was present in 30.6% and it was discordant 55%.Eber was detected in 9.3% by ISH.. The expression of immunohistochemical markers for the type of spread was not statistically significant. Conclusions: The rate of spread was the most frequently discordant with no statistical significance between the morphological and immunophenotypic subtypes. Keywords: diffuse large B cell lymphoma large infiltracción to bone marrow, immunophenotyping.