DETECCIÓN MOLECULAR DEL VIRUS DEL DENGUE EN DOS ESPECIES VECTORES HIBERNANTES COMO RESERVORIOS Y ANÁLISIS GENÉTICO DE CEPAS VIRULENTAS DE DOS ESPECIES DE HONGOS ENTOMOPATÓGENOS

Abstract

Over 50% of the flavivirus are associated with human diseases: dengue virus is one of the most important in terms of incidence of the disease. To date, there is no effective treatment against the virus and the disease control relies solely on chemicals control and community sanitation, but the vector populations are resistant to chemicals used for their control and eradication. However, there are other two important factors that contributed to the maintenance of dengue virus in nature, vertical transfer of dengue virus and the ability of vectors to hibernate (Ae. aegypti and Ae. albopictus). These two factors allow dengue virus to survive inter-epidemic stages and/or in winter, at which period the vector populations drop dramatically. It is still unknown yet at what stage of development of the hibernating vector maintains the dengue virus. Vectors sampling during the winter was conducted in the city of Rio Bravo and Reynosa, Tamaulipas. A total of 847 mosquitoes were obtained, most of which were collected as eggs and then cultured to the adult stage in the laboratory. The 830 samples of them were Ae. aegypti, 11 Ae. albopictus and 6 Culex. We analyzed 43 batches of adult mosquitoes (41pools of Ae. aegypti and 2 pools of Ae. albopictus) by RT-PCR. None of the batches tested positive (95% LCS = 0.00) using the technique of molecular detection of dengue virus. By sequencing the ITS region were molecularly identify 9 fungal isolates with possible applications in the biological control of dengue virus vectors.