Determinación de los perfiles de expresión de las moléculas reguladoras del NF-κB en tejidos gestacionales en el desarrollo del embarazo.

Abstract

The transcription factor NF-B is a nuclear factor involved in the onset of labor in late pregnancy. Because of this, NF-B should be a highly regulated molecule. During pregnancy it is suggested that early activation of this transcription factor may produce pathological effects by inducing the premature end of pregnancy, or even the loss of the fetus. Classically, the central mechanism of NF-κB regulation depends on cytoplasmic inhibitors, the IκBs. However, in recent years, they have been identified new IκBs that act regulating NF-B into the nucleus after transcriptional factor release from cytoplasmic IκBs. IκBNS is one of these non-classical NF-B regulators and it has been established that an anti-inflammatory environment promotes the expression of this regulator. In this work, using real-time PCR, we show that IκBNS can be expressed by fibroblasts cells from fetal membranes of pregnant female rats used as a study model. We also found that under a pro-inflammatory profile in rat amniochorion primary cultures cells (RACPC) is inhibited the expression of IκBNS regulator, and at the same time the expression of IL-6 is induced as a product of NF-B activation against LPS challenge. In contrast, under an anti-inflammatory profile mediated by IL-10 and in the presence of LPS, RACPC induced the expression of IκBNS and inhibited the expression of IL-6. These findings suggest that nuclear regulators, such as IκBNS, may be regulating Th-1 type cytokine production during fetal development, period that requires an anti-inflammatory profile.