Evaluación de Gen Probe Mtd-2 en la Identificación de Mycobacterium tuberculosis en Muestras Clínicas de Pacientes del Centro Médico Nacional la Raza

Abstract

Tuberculosis is a global health problem is estimated that one third of the world population is infected with a strain of Mycobacterium Tuberculosis in latency. The ability of M. latent tuberculosis to enter is one of the major challenges for global disease control. For the establishment of lung infection, microorganisms must arrive by inhalation to the alveolar spaces, which are engulfed by pulmonary macrophages complexity of this organism lies in the composition of their cell walls contain enzymes that are able to detoxify free radicals and interfere with traffic events and intracellular signaling. The initial diagnosis is clinical but the clinic is not enough for what methods should be complemented with accessories such as the tuberculin test, smear stained with Ziel- Nelsen, Lowenstein-Jensen culture is considered the gold standard and is the only method that can gain sufficient assurance accurate diagnosis of TB. This study analyzed 120 samples of patients hospitalized at the National Medical Center Gaudencio González Garza. The aim of this study was to evaluate the usefulness of direct BAT samples from patients with Mycobacterium tuberculosis search using Gen-Probe MTD-2 correlated with sputum microscopy and culture and whether it can be used as a diagnostic test for its immediate benefits provide results within 24 hours of direct samples previously subjected to decontamination and then using MTD 2 and hybridization with probes IS6110 insertion elements were stained decontaminated Ziel-Nelssen, culture and sonication, in our study we found ourselves in a low sensitivity smear as well as VPNP And for Gen Probe MTD VPNP low but with good sensitivity and specificity, we conclude that the test is useful and justified spending, limiting their use to smear positive samples.